Name:
Dr. N Chandrashekar, Scientist
eMail:
chandru.bio2020@gmail.com
 Qualification
Ph D in Molecular Biology and Biotechnology
 Date of Joining CICR 01:01:2015
Area of research
Agricultural Biotechnology
Address
ICAR - Central Institute for Cotton Research (CICR)
Post Bag No. 2, Shankar Nagar Post Office, Nagpur 440 010, Maharashtra, INDIA
Email: chandru.bio2020@gmail.com
Mobile: +91-9555339447 ;  Office: 07103-275549 (Extn: 316)
 

Academic Qualifications:

DEGREE YEAR OF COMPLETION UNIVERSITY/INSTITUTE AREA OF SPECIALIZATION
Ph D in Molecular Biology and Biotechnology 2015 Indian Agricultural Research Institute, National Institute for Plant Biotechnology, New Delhi Promoter mining and molecular characterization
M. Sc.(Agri-Plant Biotechnology) 2008 UAS, GKVK, Bangalore Drought tolerance studies
B.Sc. (Agri.) 2006 UAS, GKVK, Bangalore Agriculture

 

Employment:

Designation Institute Period From -To
Agril. Extension Officer Canara Bank 10-11-2008 to 08/07/2011
Scientist ICAR-NAARM, Hyderabad 01-01-2015 to 31-03-2015
Scientist ICAR-IARI, New Delhi 09-04-2015 to 12-06-2017
Scientist ICAR-CICR, Nagpur 14-06-2017 to Till Date

 

Area of Research:

  • Promoter mining and molecular characterization
  • Basic  studies in somatic embryogenesis in cotton
  • CRISPR/Cas9 for cotton improvement
  • Development of transgenic events resistant to bollworms in cotton

Technologies Developed/Salient Research Achievements :

  • Expression profiling of Arabidopis thaliana PR genes in response to Alternaria brassicae
  • Isolation and molecular characterization of PR-gene pathogen inducible promoter in response to Alternaria brassicae in Arabidopsis thaliana.
  • Isolation and molecular characterization of pathogenesis related PR2 gene and its promoter from Brassica juncea.
  • Isolation and characterization of osmotolerant cyanobacteria (Nostoc sp.) from Rann of kutch (Gujrat) soils.
  • Development of pMICRO12 hybrid vector from the backbones of pNEB193 and pSD5B with promoter less reporter gene (β galactosidase gene) for promoter mining in prokaryotes.
  • Induction of embryogenic callus cultures from coker genotypes of Gossypium hirsutum (Coker 310 and Coker 312) and somatic embryogenesis in cotton.
  • Molecular and biochemical analysis of UASD 78 event.
  • Isolation of Wnt-like gene from Gossypium hirsutum and development of pBI121::Wnt 3A gene construct for functional validation through plant genetic transformation studies.
  • Development of CRISPR/Cas9 gene targeting vectors for GhPHYA1

Research Projects:

Project title Funding agency Period Role
Deciphering physiological, biochemical and molecular mechanisms of abiotic stress tolerance and nutrient use efficiency of crop plants
 (ICAR-IARI, New Delhi)
ICAR 2015-2017 Co-PI
Targeted mutagenesis of GhPHYA1 through CRISPR/Cas9 in Cotton (ICAR-CICR, Nagpur) ICAR 2017-Till date PI
Basic studies on somatic embryogenesis of cotton (ICAR-CICR, Nagpur) ICAR 2017-2018 Co-PI
An efficient regeneration system for transformation studies with CICRcry2Ab1Ac and fiber strength genes in Cotton (G. hirsutum) (ICAR-CICR, Nagpur) ICAR 2017-Till date Co-PI
Unveiling the potential of cotton WNT-like gene in somatic embryogenesis through genetic engineering (ICAR-CICR, Nagpur) ICAR 2018-Till date PI
Development of Bt cotton varieties using deregulated and non deregulated transgenic events (ICAR-CICR, Nagpur) ICAR 2018-Till date Co-PI
Harnessing the potential of wild and unadapted germplasm in cotton improvement (ICAR-CICR, Nagpur) ICAR 2018-Till date Co-PI

 

Publications :

Research articles

 

International

  • Bandeppa, S., Paul, S., Jyoti, K.T., Chandrashekar, N., Deepika K.U., Chetana A., and Asha, A.D (2019). Antioxidant, physiological and biochemical responses of drought susceptible and drought tolerant mustard (Brassica juncea L) genotypes to rhizobacterial inoculation under water deficit stress. Plant Physiology and Biochemistry, 143: 19-28. https://doi.org/10.1016/j.plaphy.2019.08.018.
  • Chandrashekar, N., Ali, S. & Grover, A. (2018). Exploring expression patterns of PR-1, PR-2, PR-3, and PR-12 like genes in Arabidopsis thaliana upon Alternaria brassicae inoculation. 3Biotech. 8:230. https://doi.org/10.1007/s13205-018-1259-2
  • Ali, S., Mir, Z.A., Bhat, J.A., Tyagi, A., Chandrashekar, N., Yadav, P., Rawat, S., Sultana, M and Grover, A (2018). Isolation and characterization of systemic acquired resistance marker gene PR1 and its promoter from Brassica juncea. 3 Biotech., 8:10.https://doi.org/10.1007/s13205-017-1027-8.
  • Ali, S., Chandrashekar, N., Rawat, S. et al. (2017). Isolation and molecular characterization of pathogenesis related PR2 gene and its promoter from Brassica juncea. Biol Plant., 61(4): 763-773. https://doi.org/10.1007/s10535-017-0726-7.
  • Rawat, S., Ali, S., Nayankantha, N.N.C., Chandrashekar, N., Mittra, B and Grover, A (2017). Isolation and expression analysis of defensin gene and its promoter from Brassica juncea. J Plant Dis Prot., 124:591–600. https://doi.org/10.1007/s41348-017-0103-y.
  • Ali, S., Mir, Z.A., Tyagi, A., Bhat, J.A., Chandrashekar, N., Papolu, P.K., Rawat, S and Grover, A. (2017). Identification and comparative analysis of Brassica juncea pathogenesis-related genes in response to hormonal, biotic and abiotic stresses. Acta Physiol Plant., 39: 268.https://doi.org/10.1007/s11738-017-2565-8

National

  • N. Chandrashekar, N., Ali, S., Rawat, S. and Grover. A. (2015). Gene expression profiling of Arabidopsis thaliana chitinase genes in response to Alternaria brassicae challenge. Indian Phytopath., 68 (1) : 106-111.
  • Chandrashekar, N., Ravishankar, K. V., Laxman, R. H.,  Rekha, A. and Swarupa, V. (2012).  Identification of banana genotypes for drought tolerance. Asian J of Hort., 7(1): 13-16.
  • Chandrashekar, N., Ravishankar, K.V., Laxman, R.H., Rekha, A. and Swarupa, V. (2012). Physiological and biochemical changes during moisture stress in banana. Asian J. Bio. Sci., 7(1): 1 - 4.

Book Chapters and Conference Proceedings

  • Chandrashekar, N., Shah Vivek, Gawande, S.P and Waghmare, V.N (2020)"GC-MS analysis of methanolic extractions from Corynespora cassiicola infected cotton leaves" Published in Book of abstracts of International conference on "Advanced Functional Materials (AFM-2020) held at Kamla Nehru Mahavidyalaya, Nagpur. Page No: 56.
  • Chandrashekar, N, Raghavendra, K.P, Balasubramani, G and Waghmare, V.N (2019). Somatic embryogenesis is the necessary evil for the sustainable development of cotton through genetic engineering. Published in Book of abstracts of Global Organic Convention-2019, at Nagpur by PDKV. Akola. Page No: 174-175.
  • Saravanan, M., Chandrashekar, N and Sunil Mahajan (2019). Principles and practices of molecular phylogenetic analysis in cotton. Compendium of lectures and manual of ICAR short course on Molecular Approches for Cotton Improvement held at ICAR-CICR, Nagpur from Sep. 19th-28th, 2019. Page No:68-75.
  • Saravanan, M., Chandrashekar, N (2019). Application of MEGA software for phylogenetic inference in cotton. Compendium of lectures and manual of ICAR short course on Molecular Approches for Cotton Improvement, held at ICAR-CICR, Nagpur from Sep. 19th-28th, 2019. Page No:190-199.
  • Amudha, J., Balasubramani, G., Raghavendra, K.P., Chandrashekar, N and Waghmare, V.N (2019). Genetically modified crops for food safety, security and quality management. Published in Book of abstracts of Global Organic Convention-2019, at Nagpur by PDKV. Akola. Page No: 253-254.
  • Balasubramani, G., Amudha, J., Raghavendra, K.P., Chandrashekar, N and Waghmare, V.N (2019). Biosafety measures on genetically modified crops in India. Published in Book of abstracts of Global Organic Convention-2019, at Nagpur by PDKV. Akola. Page No: 258-259.
  • Raghavendra, K.P., Chandrashekar, N., Amudha, J., Balasubramani, G and Waghmare, V.N (2019). Detection of herbicide tolerant transgenes and events to minimize GM contamination in organic cotton. Published in Book of abstracts of Global Organic Convention-2019, at Nagpur by PDKV. Akola. Page No: 290-291.
  • Chandrashekar, N., Raghavendra,K.P., Balasubramani G and V.N.Waghmare. Gene Delivery Techniques and Genetic Enhancement of Cotton. In book of Abstracts international conference on multifunctional advanced material (ICMAM-2018) Nagpur, pp: 44.
  • Raghavendra,K.P, Rakesh Kumar, Chandrashekar N. Vivek Shah, Balasubramani G. and V.N.Waghmare. Detection of Genetically Modified Events in Cotton. In book of abstracts international conference on multifunctional advanced material (ICMAM-2018) Nagpur, pp: 46.
  • Chandrashekar, N and Dhar, D.W (2017). Isolation of osmotolerant cyanobacteria for enhancing drought tolerance in rice. International conference on “Recent trends in science and technology: Sustainable development through scientific approach” organized by Shri Shivaji Education Society, held at Shri Shivaji Education Society’s Science College, Congress Nagar, Nagpur, Maharashtra from 12th-14th july-2017. IJRBAT, Special Issue, Vol-V, July 2017. Page No-117: Abstracts:ICRTS-2017:ISSN No:2347-517X.
  • Joy Das, Santosh, H.B., Rakesh Kumar, Chandrashekar, N and Raghavendra K.P. CRISPR/CAS technology for next-generation cotton: An overview. Proceedings of Seventh Asian Cotton Research and Development Network (ACRDN): Meeting, Sept 15-17, 2017 at Hotel Le Meridien, Nagpur, Maharashtra, India.
  • Chandrashekar, N., Sajad Ali and Anita Grover (2016). Expression patterns of PR-1, PR-2, PR-3 and Plant defensin genes in Arabidopsis thaliana upon Alternaria brassicae inoculation. O(S4B)026: IPS 6th International conference on “Plant, Pathogens and People” organised by Indian phyto pathological society, Division of plant pathology, IARI, New Delhi, held at NASC complex from 23-27th February-2016. Page No:44, ISBN:81-7019-527-2.
  • Vankudothu Ravinder Naik, Rabindra Padaria, N. Chandrashekar and N. Naresh Babu. 2012 (Feb) Mobile—A Catalyst in the Transfer of Agriculture Technology. Proceedings of M4D 2012, New Delhi.
  • Naresh Babu, N., Nandini C., Chandrashekar, N., Shailaja Hittalmani., 2011(Sep), Yield Potential and Drought Susceptibility Index of Promising Aerobic Rice (Oryza sativa L.) Genotypes under moisture stress. National symposium on “Advances in Biotechnological Research in Agri-Horticultural Crops for Sustaining Productivity, Quality Improvement & Food Security” Sardar Vallabhbhai Patel University of Agri. & Tech., Meerut- 250110 U.P
  • Naresh Babu N., Nandini C., Chandrashekar, N., Shailaja Hittalmani., 2011(Sep).Validation of marker linked to fertility Restoration (Rf) locus in Rice (Oryza sativa L.) under transplanted and Aerobic condition.” National symposium on “Advances in Biotechnological Research in Agri-Horticultural Crops for Sustaining Productivity, Quality Improvement & Food Security” Sardar Vallabhbhai Patel University of Agri. & Tech., Meerut- 250110 U.P.
  • Nandini C, Asif, M, Chandrashekar, N., Naresh Babu, N., Puspa D, Sandya M. K. 2011(Oct), Breeding for Water use efficiency. National conference on Recent Trends in Biosciences, Organised by Dept of Biochemistry and biotechnology, Sri Pratap College. Srinagar.

Paper Presented in Conference/seminar/symposia (National / International)

  • International
    • "GC-MS analysis of methanolic extractions from Corynespora cassiicola infected cotton leaves" during international conference on "Advanced Functional Materials (AFM-2020) held at Kamla Nehru Mahavidyalaya, Nagpur from 23rd-25th, Jan. 2020.
    • Gene Delivery Techniques and Genetic Enhancement of Cotton. International Conference on Multifunctional Advanced Materials (ICMAM) organized by Kamla Nehru Mahavidyalaya, held from October 5 to 7, 2018 at Hotel Ganga Kashi, Jagnade Square, Nagpur.
    • Isolation of osmotolerant cyanobacteria for enhancing drought tolerance in rice. International conference on “Recent trends in science and technology: Sustainable development through scientific approach” organized by Shri Shivaji Education Society, held at Shri Shivaji Education Society’s Science College, Congress Nagar, Nagpur, Maharashtra from 12th-14th july-2017.
    • Expression patterns of PR-1, PR-2, PR-3 and Plant defensin genes in Arabidopsis thaliana upon Alternaria brassicae inoculation. International conference on “Plant, Pathogens and People” organised by Indian phyto pathological society, Division of plant pathology, IARI, New Delhi, held at NASC complex from 23-27th February-2016.
  • National: NIL

Popular Articles/ Short Notes/ Short Communications

  • Raghavendra, K. P., Rakesh Kumar, Joy Das and Chandrashekar, N. 2018. PCR based detection of transgenic events in GM cotton. Lecture notes: ICAR Sponsored winter school- Insect resistance to Bt toxins and Insecticides in cotton. Jan 18-Feb 07, Nagpur, India. Pp: 132.
  • Raghavendra, K. P., Madhu, T. N and Chandrashekar, N. 2018. Bt protein evolution checks the development of insect resistance in GM crops. Lecture notes: ICAR Sponsored winter school- Insect resistance to Bt toxins and Insecticides in cotton. Jan 18-Feb 07, Nagpur, India. Pp: 141.
  • Raghavendra, K. P., Rakesh Kumar, Joy Das and Chandrashekar, N. 2018. PCR based detection of transgenic events in GM cotton. Practical mannual: ICAR Sponsored winter school- Insect resistance to Bt toxins and Insecticides in cotton. Jan 18-Feb 07, Nagpur, India. Pp: 13-14.
  • N. Naresh Babu, N. Chandrashekar, Erayya, 2012(June). Success story of development of QPM and its Nutritional Impact. Agrobios Newsletter, 9(1):70-71.
  • N. Chandrashekar, N. Naresh Babu, Erayya, 2012(June). Plants as bioreactors for the production of Pharmaceuticals. Agrobios Newsletter, 9(1):61.
  • G. S. Panduranga, N. Chandrashekar, P. Krishna Reddy, N. Naresh Babu, 2012(November). Adipokinetic hormone: A lipid mobilising hormone in insects. Agrobios Newsletter, 9(6):77-78.

Training Manual

  • Involved in generating “Training manual on GM Cotton Testing”   conducted for seed testing officers from different states held at ICAR-CICR, Nagpur from 3rd-7th February, 2020 and from 2nd-7th, March, 2020

Research Reports / Annual Reports:

  • Served as member for editorial committee of the institute annual report 2017-18

Web Portals/ E-Publications/ CD Development/ Mobile App

  • B. L. Patil and N. Chandrashekar (2017). Teaching review on “Strategies for Abiotic Stress Tolerance in Plants” as part of Syllabus on “Plant Genetic Engineering” for e-Pathshala by UGC-INFLIBNET (University Grants Commission-Information and Library Network Centre, India).
  • B. L. Patil, N. Chandrashekar and Siddanna Savadi (2017). Teaching review on “Strategies for Resistance to Fungal Pathogens” as part of Syllabus on “Plant Genetic Engineering” for e-Pathshala by UGC-INFLIBNET (University Grants Commission-Information and Library Network Centre, India).
  • Pooja verma, Savitha Santosh and Chandrashekar N. (August, 2017). SWEET gene: Paving the way towards re-emergence of bacterial blight in cotton. Cotton Innovate: 8(3):1. Available at www.cicr.org.in.
  • N. Chandrashekar, S. ALI and A. GROVER* (2017). Cultivation of Arabidopsis thaliana the Model Plant for Genetic Modification and Molecular Biology Applications:Biotecharticles. Available online at http://www.biotecharticles.com/Agriculture-Article/Cultivation-of-Arabidopsis-Thaliana-3898.html https://www.biotecharticles.com/Agriculture-Article/Cultivation-of-Arabidopsis-Thaliana-3898.html
  • Chandrashekar, N., Raghavendra, K.P and Santosh, H.B. (October, 2017). CRISPR-Cas9 technology for targeted genome editing in cotton. Cotton Innovate: 10(3):1. Available at www.cicr.org.in

Seminar/Symposia/Conference/Workshop/Meeting Attended :

 

Conferences:

  • International conference on “Plant, Pathogens and People” organized by Indian phyto pathological society, Division of Plant Pathology, IARI, New Delhi, held at NASC complex from 23-27 th February-2016.
  • International conference on “Recent trends in science and technology: Sustainable development through scientific approach” organized by Shri Shivaji Education Society, held at Shri Shivaji Education Society’s Science College, Congress Nagar, Nagpur, Maharashtra from 12th-14th july-2017.
  • International Conference on Multifunctional Advanced Materials (ICMAM) organized by Kamla Nehru Mahavidyalaya, held from October 5th to 7 th, 2018 at Hotel Ganga Kashi, Jagnade Square, Nagpur.
  • Global Organic Convention-2019 on “Natural Resource Management for Sustainable Agriculture, Soil health and Quality Food”, 15th-17th Sept. 2019, organised by Centre for Organic Agricultural Research and Training and Society of Agriculture Research and Development, Dr. P.D.K.V., Akola, India held at Le Meridien Hotel, Nagpur.
  • International conference on "Advanced Functional Materials (AFM-2020) held from January, 230 rd-25 th, 2020 at Kamla Nehru Mahavidyalaya, Nagpur, Maharashtra India.

Workshops:

  • Workshop on " PGPRs for sustainable Crop Productivity" Organised by Indian Phytipathological Society, held at NASC, Complex on 25-Feb-2016 DPS Marg, New Delhi.
  • Workshop on DST’s ‘’Knowledge network on climate and agriculture’’ Organised by Department of Science and Technology, Government of India and IARI, New Delhi from April 28-29, 2016 held at NASC Complex, DPS Marg, New Delhi.

 

  • Indo-US Bilateral workshop on “Genomic approaches for yield enhancement and biological nitrogen fixation in Chickpea’’ organised by IARI New Delhi from 29- 31 st January 2017 at NASC complex New Delhi.

Meetings:

  • Seventh Asian Cotton Research and Development Network (ACRDN) Meeting
    Sept 15-17, 2017 at Hotel Le Meridien, Nagpur, Maharashtra, India.

Training Attended/Training Conducted/Invited Lectures :

Training Attended

National

  • Professional attachment training as a part of FOCARS 101 at Indian Institute of Science, Division of Biochemistry, Bengaluru from 21.05.2015 to 20.8.2015.
  • ICAR sponsored CAFT training programme on "Plant Omics – Emerging Tools and Techniques for crop improvement” held from 18.11.2015 to 8.12.2015 at Division of Biochemistry , Pusa Campus, New Delhi.
  • Training programme on “Radioisotope Use and Safety” organized by the radiological safety office, IARI at the nuclear research laboratory, IARI, New Delhi held from 10th-11th march, 2016.
  • ICAR sponsored CAFT training programme on “Computational and statistical advances for analysis of biological data in agriculture” held from 24.03.2018 to 13.04.2018 at ICAR-IASRI, New Delhi.
  • ICAR sponsored short course on “Molecular Approaches for Cotton Improvement” held from 19.09.2019 to 28.09.2019 at ICAR-CICR, Nagpur.

Training Conducted:
As a course co-ordinator:

  • Three days training program on "Molecular techniques  for transgene detection in GM cotton" for seed testing officers from state agriculture department of Maharashtra was conducted from 16 to 18th October 2017 at Biotechnology laboratory, Division of Crop Improvement, ICAR-CICR, Nagpur.

 

  • Two trainings were conducted for state seed testing officials from different states of India on “GM cotton testing” from 3rd-7th, February, 2020 and 2nd-7th, March, 2020, at ICAR-CICR, Nagpur

Invited Lectures;

Delivered an invited lecture on “Exploration of CRISPR/Cas9 Genome Editing Technology for Cotton Improvement” during ICAR short course on Molecular Approaches

 

Other Assignments and Membership:

ASSOCIATION IN PROFESSIONAL SOCIETIES

  • Life time member of Indian society of cotton improvement (ISCI)

OTHER OFFICAL ASSIGNMENTS

  • Member of Institute Library committee ICAR-CICR, Nagpur
  • Member of institute MGMG-Umred cluster

CONSULTANCIES PROVIDED: NIL

 

I certify that the above information is correct to the best of my knowledge - Dr. N. Chandrashekar

Page created by : Dr. M Sabesh; Page created in 2020; Last updated on 30:06:2020